Cambridge Healthtech Institute’s 20th Annual

Cell Line Engineering and Cell Culture Optimization

Improving Productivity and Product Quality

August 19-20, 2024


The field of cell line development and cell culture are undergoing a revolution, driven by technological advancements and a critical need for efficient, high yield biopharmaceutical production. We are entering the era of precision cell engineering, where CRISPR-gene editing, targeted integration, and next generation sequencing are sculpting cell lines for enhanced stability productivity and protein quality, while AI and machine learning are analyzing vast datasets to potentially predict optimal bioreactor conditions, mimic microenvironments, and anticipate bottlenecks. Join our faculty of experts to hear about their current challenges, and their vision for the future of Cell Line Engineering and Cell Culture Optimization.


Coverage will include, but is not limited to:



  • Host cell engineering
    • CRISPR-directed gene editing
    • Site-specific targeted integration vs random integration
    • Transposon-based random integration
    • Developing novel vectors and landing sites for precise control of gene expression and genomic integrity
  • Proteomic analysis of CHO cell clones to improve phenotypes
  • Implementing high throughput screening and selection methods for rapid identification of optimal clones
  • Using AI, NGS, and predictive models to guide cell line development and clone selection for best producers
  • Alternative host cells beyond CHO
  • Humanized cell lines for improved compatibility and reduced immunogenicity
  • Engineering cell lines for expression of complex biologics like bispecific antibodies and multimeric proteins
  • Designing cell lines with robust growth and protein production capabilities for large-scale bioreactors
  • Integrating cell line development with downstream processing and purification steps
  • Stable cell line development for gene therapy products


  • High cell density culture
  • Cell-free DNA production method
  • Codon optimization
  • Scaling up and Reproducibility - transitioning from small scale to large scale bioreactors
  • Automation and robust process control to minimise batch-to-batch variability
  • Enhancing protein expression and productivity
  • Optimizing media composition and feeding strategies
  • Addressing post-translational modifications like glycosylation and aggregation
  • Monitoring and controlling cellular stress responses
  • Developing in-line analytical tools for real-time monitoring of protein attributes
  • Using machine learning and advanced modeling to predict optimal culture conditions
  • Integrating microfluidic and other platforms for improved cell culture models
  • Implementing novel bioreactor designs and optimization strategies for energy efficiency
  • Developing sustainable waste management solutions for used culture media and cells

The deadline for priority consideration is February 2, 2024.


All proposals are subject to review by session chairpersons and/or the Scientific Advisory Committee to ensure the overall quality of the conference program. Additionally, as per Cambridge Healthtech Institute’s policy, a select number of vendors and consultants who provide products and services will be offered opportunities for podium presentation slots based on a variety of Corporate Sponsorships.


Opportunities for Participation:



For more details on the conference, please contact:

Mimi Langley

Senior Conference Director

Cambridge Healthtech Institute

Phone: (+1) 781-972-5439



For sponsorship information, please contact:

Companies A-K

Phillip Zakim-Yacouby

Senior Business Development Manager

Cambridge Healthtech Institute

Phone: (+1) 781-247-1815



Companies L-Z

Aimee Croke

Business Development Manager

Cambridge Healthtech Institute

Phone: (+1) 781-292-0777