The Bioprocessing Summit

Cambridge Healthtech Institute’s Inaugural
Advances in Purification Technologies
Advanced Technologies and Novel Concepts in Protein Purification
Part of CHI's 6th Annual The Bioprocessing Summit

August 21-22, 2014 | Renaissance Waterfront Hotel | Boston, Massachusetts


While upstream process scientists are churning out higher and higher titers, downstream process engineers have been busy with developing possible solutions to ameliorate the bottlenecks, increase productivity while lowering cost. Some of these technological advances are novel concepts while others are radical advances that push the envelopes. CHI’s Advances in Purification Technologies conference offers insights into the exciting world of purification driven by developments in chromatography, crystallization, precipitation, filtration and clarification. This is a platform for you and your colleagues to come together to exchange ideas and review the innovations in downstream purification.

Fast-track your learning objectives by attending the co-located Short Course on ABC: Anything but Chromatography – Precipitation, Crystallization and Flocculation. This 3-hour dinner short course is conducted by Dr. Alois Jungbauer of BOKU to help you overcome productivity limits and design a cost-effective scalable process.


Day 1 | Day 2 | Short Courses | Download Brochure | Speaker Bios

Thursday, August 21

1:55 pm Chairperson’s Remarks

Greg Zarbis-Papastoitsis, Ph.D., Vice President, Process and Manufacturing Sciences, Eleven Biotherapeutics


2:00 keynote PRESENTATION

Cold Ethanol Precipitation and Flocculation for Continuous Downstream Processing of Recombinant Antibodies

Alois JungbaerAlois Jungbaer, Ph.D., Professor, Department of Biotechnology, University of Natural Resources and Life Science Vienna, (BOKU) and Austrian Centre of Industrial Biotechnology

We have developed a completely new, non-chromatographic alternative based on a series of selective precipitation and flocculation steps. Our process does not need elution chromatography anymore. The method is a generic platform technology and has been tested in the purification of several human antibodies with different pI and hydrophobicity. The full potential of this new continuous downstream technology can be harnessed by coupling to a continuous upstream process/perfusion reactor. Examples for fully continuous operating reactors will be shown.


NEW APPROACHES AND TECHNOLOGIES IN PURIFICATION CHROMATOGRAPHY

2:45 Dramatic Improvements in Process Economy with Non-Column Purification

Richard Nian, Ph.D., Research Scientist, Downstream Processing Group, Bioprocess Technology Institute, A*Star, Singapore

In this study, we show data from new clarification methods that enable a number of non-protein A capture alternatives, including non-column formats. We also show data from a breakthrough convection-based system that supports 10-fold lower host protein, aggregate and DNA content, plus 10-20% higher IgG recovery compared to 3-step protein A platforms. And it achieves these results with two thirds less water than current protein A platforms.

3:15 A Designed Calcium-Responsive Peptide Domain for Non-Chromatographic Protein Purification

Scott Banta, Ph.D., Associate Professor, Chemical Engineering, Columbia University

We have discovered a peptide sequence based on the beta-roll forming RTX domain that reversibly precipitates in the presence of calcium. We have developed this phase-change as a simple method for the non-chromatographic purification of recombinant proteins. We have demonstrated this technique with several model proteins, and the technique should be broadly applicable.

3:45 Sponsored Presentation (Opportunity Available)

4:00 Refreshment Break in the Exhibit Hall with Poster Viewing

4:45 Breakout Discussions

This session provides the opportunity to discuss a focused topic with peers from around the world in an open, collegial setting. Select from the list of topics available and join the moderated discussion to share ideas, gain insights, establish collaborations or commiserate about persistent challenges. At the end of the session, each moderator will summarize the topics being discussed, the findings and conclusions (if any), and share with the audience.

5:45 End of Day


Suggested Short Course*

5:45 – 6:30 Dinner Short Course Registration

6:30 – 9:00 Dinner Short Course*: ABC: Anything But Chromatography – Precipitation, Crystallization and Flocculation 


*Separate registration required


Day 1 | Day 2 | Short Courses | Download Brochure | Speaker Bios