Cambridge Healthtech Institute’s 10th Annual
Optimizing Cell Culture Technology
Enhancing Knowledge for Growing Cells
Part of CHI's 6th Annual The Bioprocessing Summit
August 18-19, 2014 | Renaissance Waterfront Hotel | Boston, Massachusetts
This leading cell culture meeting explores today’s evolving strategies and technologies for improving mammalian cell cultivation, including the omic sciences, such as metabolomics and genomics. Industry experts offer insights into optimizing conditions as well as cell biology in an effort to improve yield, while also addressing where cell culture is heading as it meets ever greater production demands and quality standards.
The agenda again features a session focused on CHO (Chinese Hamster Ovary cells), as well as small-group breakout discussions that provide the opportunity to discuss important topics with your peers from around the world.
Day 1 | Day 2 | Short Courses | Download Brochure
Monday, August 18
8:00 am Pre-Conference Registration and Morning Coffee
11:30 Main Conference Registration
1:00 pm Chairperson’s Opening Remarks
Lada Laenen, Ph.D., Head, Cell Culture and Microbiology, MSAT, Genzyme, a Sanofi Company
1:10 OPENING KEYNOTE PRESENTATION:
The Future of Cell Culture Technology
Bert Frohlich, Ph.D., Director, Bioengineering, Shire Human Genetic Therapies
This talk will begin with a brief overview of factors influencing the direction of cell culture technology and those shaping the biopharmaceutical industry. The overview will serve to tie together many of the subjects covered in this conference. Emphasis will then shift to large-scale production of recombinant proteins and the increasing need for control of product quality and consistency. Emerging technologies, quality-by-design and tools for optimization and achieving improved process understanding will also be touched upon.
1:45 A Statistical Approach to Enhance Productivity in Cell Culture Fed Batch Processes
Hanuman Mallubhotla, Ph.D., Research Director and Head, Biopharmaceutical Development, Syngene International, Ltd.
A Design-of-Experiments (DoE) methodology was developed in deriving optimal basal media, feed media and process parameter settings for a cell culture process. Fifteen basal media and seven feed media were screened; feed rate and temperature conditions were optimized based on statistically observed interaction profiles as well as amino acid profiles. Through optimized feed rate and biphasic-temperature culture conditions, the titer was increased by > 6.0-fold from ~ 0.5g/L in shake flasks to > 3.0 g/L in bioreactors.
2:15 The Application of Systems Biology in Bioprocess Optimization
Len van Zyl, Ph.D., CEO and CSO, ArrayXpress, Inc.
The integration of a Systems Biology approach to optimize and speed-up upstream and downstream bioprocesses is gaining significant traction in the biopharmaceutical industry. Improving our understanding of the cells, the actual bioreactors themselves, provides for a beginning to end development approach to improve product quality and performance. Systems biology as a concept aims to map all conceivable interactions within a system through a set of measurable variables.
2:45 Refreshment Break
3:15 Antibody Screening in Mammalian Suspension Cells
Michael R. Dyson, Ph.D., Senior Research Associate, Biochemistry, University of Cambridge, and Group Leader, IONTAS, Ltd.
An important step in the process of recombinant antibody selection and optimisation by phage display is the conversion to IgG and Fab format and multi-parallel expression in mammalian suspension cells. This is to both select for clones that can be expressed in high yield and provide antibodies for cell-based functional assays. Methods will be presented for high-throughput antibody expression in HEK293, CHO and stem cells including case studies for the selection of functionally active antibodies.
3:45 Modulation of the Quality Attributes of a Monoclonal Antibody Using Micro-L Scale Fed-Batch Cultures
Matthieu Stettler, Ph.D., Manager, Upstream Development, Biotech Process Sciences, Merck Serono SA
A high-throughput DoE approach was used to explore the impact of media and feed components on the main quality attributes of a monoclonal antibody. The experiment was performed using a new cultivation system based on shaking 96-deepwell plates. This integrated early cell culture process development approach was found to be particularly fast and resource efficient and the outcome correlated ideally with confirmations performed in larger cell culture volumes such as shake tubes and small-scale bioreactors.
4:15 Small Group Breakout Discussions
This session provides the opportunity to discuss a focused topic with peers from around the world in an open, collegial setting. Select from the list of topics available and join the moderated discussion to share ideas, gain insights, establish collaborations or commiserate about persistent challenges. Then continue the discussion as you head into the lively exhibit hall for information about the latest technologies.
5:15 Discussion Report-Outs
5:30 Grand Opening Reception in the Exhibit Hall with Poster Viewing
7:00 End of Day
Day 1 | Day 2 | Short Courses | Download Brochure