The Bioprocessing Summit
The Bioprocessing Summit

CONFERENCE SERIES: Bioprocessing & Manufacturing


Digital Course: Optimizing Media: Achieving Super Soup


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About this Product: 

The “Optimizing Media” digital course provides a detailed overview of the process for successfully developing media for culturing cells, with an emphasis on providing optimal conditions for cell growth. The overview addresses media supplementation, the importance of amino acids, protein free media, and serum versus serum-free media. In addition, the digital course includes a discussion about developing a chemically-defined basal and feed media for fed-batch cell culture, which includes strategies for optimizing basal and feed media, and developing fed-batch platforms. This presentation also highlights the use of Design of Experiments (DoE) strategies for developing basal media.

The strategies being covered:

  • Defines the basic requirements of cell culture media, and how to achieve success
  • How to apply principles of DoE for optimizing cell culture media
  • Best practices for developing media for fed batch cell culture



Product Details:
2 Presentations
62 Slides
Total Run Time: 58:39
Digital Download: $345.00
Single Copy: $345.00
Site License: $1380.00

Formats Available:
Digital Download
On Demand 


Agenda at a Glance: 

Fundamental Aspects to Successful Media Optimization

Kamal Rashid, Ph.D., Director, Biomanufacturing Education and Training Center, Worcester Polytechnic Institute

Media composition plays a significant role in cell yield and viability in the culture. Yield and viability are very important factors in productivity of cell-based processes for biologics production. Animal cell culture media contains a mixture of amino acid, vitamins, glucose, salts and other nutrients such as growth hormones and growth factors. The requirements for these nutrients vary from one cell line to another making optimization studies an absolute necessity for individual cell lines.

In this presentation, an overview of media for cell culture will be discussed with emphasis on:

  • The importance of media for cell growth
  • Media composition
  • Media supplementations
  • The importance of amino acids in the media
  • Serum and serum free media
  • Protein free media

Development of Chemically-Defined Basal and Feed Media for a Fed Batch Cell Culture Platform

Seshu Tummala, Ph.D., Senior Scientist, Manufacturing Sciences and Technology Group, Lonza Biologics, Inc.

A chemically defined fed-batch platform process for biotherapeutic protein production has the benefits of decreasing cost of goods via media cost reduction and improving process understanding through knowledge of the effects of individual media components on process performance. In addition, such processes can lead to decreased time for generation of clinical grade material and facilitate the use of Quality-by-Design principles which will lead to faster regulatory agency approval. In this case study, simple basal and fed-batch media strategies to develop a chemically defined fed-batch platform will be discussed.

Presentation will include:

  • Definition of key groups of media components
  • Use of DOE in basal media development
  • Strategies for optimizing basal and feed media
  • Development of fed batch platforms

Speaker Biographies: 

 KamalRashidKamal Rashid, Ph.D., Director, Biomanufacturing Education and Training Center, Worcester Polytechnic Institute

I have over thirty years of academic experience in both research and Biotechnology educational program development. During my career I have developed, directed and implemented biotechnology training courses at Utah State University, Penn State University and internationally. I joined Utah State University in July 2000 as the Biotechnology Center’s Associate Director and Research Professor of Toxicology. During my tenure at Utah State University, I developed and equipped the bioprocess facility at the Center with the most advanced bioreactors and fermenters that are utilized in both research and training programs. While at Utah State University I received a multi-year, multimillion dollar grant from the US Department of Health and Human Services Biomedical Advanced Research and Development Authority (BARDA) to train employees of vaccine manufacturing facilities from eleven countries in the latest advances in cell-based vaccine production with emphasis of Influenza vaccines. These countries included Brazil, Egypt, Kazakhstan, Korea, India, Indonesia, Mexico, Romania, Russia, Serbia, South Africa, Thailand and Vietnam.

Prior to joining USU, I was a faculty member in the Department of Biochemistry and Molecular Biology at the Pennsylvania State University. While at Penn State, I conducted research on the impact of environmental pollutants on human health, developed and taught biotechnology undergraduate courses, developed and directed the Penn State biotechnology training programs, directed the Summer Symposium in Molecular Biology for ten years and was the key faculty in the development of the biotechnology undergraduate degree and the course curriculum in the department. I have delivered numerous lectures and training programs in several countries, including Canada, China, Dominican Republic, Egypt, Indonesia, Iraq, Korea, Malaysia, Philippines, Puerto Rico, Vietnam, Thailand, Taiwan, Singapore and US. I am recognized for my continuing education, teaching and international programs. I received a national Faculty Service Award in 1997 from US University Continuing Education Association for my “meritorious service to Penn State University”. I was also honored in 2011 as the international professor of the year in College of Agriculture at Utah State University.

 SeshuTummalaSeshu Tummala, Ph.D., Senior Scientist, Manufacturing Sciences and Technology Group, Lonza Biologics, Inc.

Seshu Tummala, Ph.D., is currently a Senior Scientist in the Manufacturing Sciences and Technology group at Lonza Biologics, where his main responsibilities are scale-up, tech transfer, and process troubleshooting. He gained his expertise with media development via various roles in process development and manufacturing sciences at institutions including Abbott Laboratories, Percivia, Sanofi Pasteur, Alnylam, as well as Lonza Biologics. He received his BS in Chemical Engineering from Johns Hopkins University and MS and PhD in Chemical Engineering from Northwestern University. He has published in journals related to bioprocessing including Biotechnology & Bioengineering and Biotechnology Progress and has presented at numerous conferences in the biotech field.

About the Conference: 

The Bioprocessing Summit brings together international leaders to discuss today’s bioprocess issues from cell line selection to bioproduction. The Summit provides practical details in a relaxed, congenial atmosphere that promotes information exchange and networking.

This leading bioprocess meeting is hosted in Boston each summer along the lively and cosmopolitan harbor waterfront. Hundreds of bioprocess professionals come together each year at the Summit to share practical solutions for today’s bioprocess challenges with researchers from around the world.

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Cell Culture Scientist
Senior Scientist
Lab Manager
Director, Cell Culture
Senior Director, Cell Culture
Associate Director
Cell Culture Process Development
Scientific Director
Product Manager