Archived Content


SC 1 - OPTIMIZING MEDIA: Achieving Super Soup 

Monday, August 18 | 9:00 to 11:30am



9:00 Chair Remarks

Yuan Wen, Ph.D., Process Science Manager, Gibco PD-Direct Bioprocess Services, Thermo Fisher Scientific

9:05 The Importance of Media in Cell Culture Processes

Kamal RashidKamal Rashid, Ph.D., Director, Biomanufacturing Education and Training Center, Worcester Polytechnic Institute 

Media composition plays a significant role in cell yield and viability in the culture. Yield and viability are very important factors in productivity of cell-based processes for biologics production. Animal cell culture media contains a mixture of amino acid, vitamins, glucose, salts and other nutrients such as growth hormones and growth factors. The requirements for these nutrients vary from one cell line to another making optimization studies an absolute necessity for individual cell lines. In this presentation an overview of media for cell culture will be discussed with emphasis on: 


• The importance of media for cell growth
• Media composition
• Media supplementations
• The importance of amino acids in the media
• Serum and serum free media
• Protein free media

9:30 Development of Chemically-Defined Basal and Feed Media for a Fed Batch Cell Culture Platform

Seshu TummalaSeshu Tummala, Ph.D., Senior Scientist, Manufacturing Sciences and Technology Group, Lonza Biologics, Inc.

A chemically defined fed-batch platform process for biotherapeutic protein production has the benefits of decreasing cost of goods via media cost reduction and improving process understanding through knowledge of the effects of individual media components on process performance.  In addition, such processes can lead to decreased time for generation of clinical grade material and facilitate the use of Quality-by-Design principles which will lead to faster regulatory agency approval.   In this case study, simple basal and fed-batch media strategies to develop a chemically defined fed-batch platform will be discussed. 

Presentation will include:

  • Definition of key groups of media components
  • Use of DOE in basal media development
  • Strategies for optimizing basal and feed media
  • Development of fed batch platforms

10:00     Break


10:30     Fast, Easy, and Reliable Methods for Quantifying Cell Culture Media Variance and Stability:  Or…How To Taste Your Super Soup…..  

Alan G. RyderAlan G. Ryder, Ph.D., Senior Lecturer, Nanoscale Biophotonics Laboratory, School of Chemistry, National University of Ireland-Galway 

The complex composition of cell culture media means that media are inherently unstable, for example, visible light exposure causes extensive photo-damage which in turn adversely affects cell growth.  This can be significant when transparent bioreactors/media containers are used.  Here we show rapid and effective monitoring of cell culture media variance and stability using Fluorescence excitation-emission matrix spectroscopy combined with multi-way chemometrics and/or Surface Enhanced Raman Scattering (SERS).  First, for monitoring media photo-damage (riboflavin-based degradation pathways), and second for identifying media decomposition under normal media storage conditions.

11:00     Extended Discussion / Q&A 

11:30     Close of Optimizing Media Short Course  



Speaker Biographies: 


Kamal Rashid, Ph.D., Director, Biomanufacturing Education and Training Center, Worcester Polytechnic Institute  

I have over thirty years of academic experience in both research and Biotechnology educational program development. During my career I have developed, directed and implemented biotechnology training courses at Utah State University, Penn State University and internationally. I joined Utah State University in July 2000 as the Biotechnology Center’s Associate Director and Research Professor of Toxicology. During my tenure at Utah State University, I developed and equipped the bioprocess facility at the Center with the most advanced bioreactors and fermenters that are utilized in both research and training programs. While at Utah State University I received a multi-year, multimillion dollar grant from the US Department of Health and Human Services Biomedical Advanced Research and Development Authority (BARDA) to train employees of vaccine manufacturing facilities from eleven countries in the latest advances in cell-based vaccine production with emphasis of Influenza vaccines. These countries included Brazil, Egypt, Kazakhstan, Korea, India, Indonesia, Mexico, Romania, Russia, Serbia, South Africa, Thailand and Vietnam.

Prior to joining USU, I was a faculty member in the Department of Biochemistry and Molecular Biology at the Pennsylvania State University. While at Penn State, I conducted research on the impact of environmental pollutants on human health, developed and taught biotechnology undergraduate courses, developed and directed the Penn State biotechnology training programs, directed the Summer Symposium in Molecular Biology for ten years and was the key faculty in the development of the biotechnology undergraduate degree and the course curriculum in the department. I have delivered numerous lectures and training programs in several countries, including Canada, China, Dominican Republic, Egypt, Indonesia, Iraq, Korea, Malaysia, Philippines, Puerto Rico, Vietnam, Thailand, Taiwan, Singapore and US. I am recognized for my continuing education, teaching and international programs. I received a national Faculty Service Award in 1997 from US University Continuing Education Association for my “meritorious service to Penn State University”. I was also honored in 2011 as the international professor of the year in College of Agriculture at Utah State University.

Seshu Tummala, Ph.D., Senior Scientist, Manufacturing Sciences and Technology Group, Lonza Biologics, Inc. 

Seshu Tummala, Ph.D., is currently a Senior Scientist in the Manufacturing Sciences and Technology group at Lonza Biologics, where his main responsibilities are scale-up, tech transfer, and process troubleshooting.  He gained his expertise with media development via various roles in process development and manufacturing sciences at institutions including Abbott Laboratories, Percivia, Sanofi Pasteur, Alnylam, as well as Lonza Biologics.  He received his BS in Chemical Engineering from Johns Hopkins University and MS and PhD in Chemical Engineering from Northwestern University.  He has published in journals related to bioprocessing including Biotechnology & Bioengineering and Biotechnology Progress and has presented at numerous conferences in the biotech field.

Alan G. Ryder, Ph.D., Senior Lecturer, Nanoscale Biophotonics Laboratory, School of Chemistry, National University of Ireland-Galway 

Dr. Alan G. Ryder is a Senior lecturer in the School of Chemistry at the National University of Ireland, Galway (NUIG).  He has a B.Sc. and Ph.D. in chemistry from NUIG.  After a stint as a postdoctoral researcher in UCC, he rejoined NUIG in 1997 to work on developing quantitative Raman spectroscopy based methods for measuring illicit narcotics.  In 2003 he formed the Nanoscale Biophotonics Laboratory (NBL) which is focused on the use of photonics and chemometrics technologies for life and physical science applications.  In 2006 obtained tenure in the School of Chemistry at NUIG where he lectures mainly in spectroscopy and has two core research areas: Analytical Sciences and Photonics which cover a wide range of applications.  In the Analytical Sciences domain the main research area is the development of rapid, quantitative analytical methods for the analysis of complex materials with particular focus on biopharmaceutical manufacturing.  Key methods employed include Raman and Fluorescence spectroscopies and chemometrics.  He has collaborated with a range of industry partners including Bristol-Myers Squibb, Janssen-Biologics, Merck, Agilent, and Kaiser Optical Systems.  He has authored in excess of 70 publications, generated 3 patents, graduated 11 PhD students.

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