Cambridge Healthtech Training Seminars

BASIC TECHNOLOGIES IN A PROTEIN PRODUCTION LAB

MONDAY, AUGUST 21 – TUESDAY, AUGUST 22, 2017
DAY 1: 1:00PM - 5:00PM | DAY 2: 8:00AM - 5:15PM


Instructors:

Tsafi Danieli, Ph.D., Director, BioGiv Excubator & Head, Protein Expression Facility, Wolfson Centre for Applied Structural Biology, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem

Mario Lebendiker, Ph.D., Head, Protein Purification Facility, Wolfson Centre for Applied Structural Biology, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem
July 2017 Speaker Interview 


Do you work at an early stage, biotech startup focusing on protein expression and therapeutics? Are you responsible for building and running a protein production lab and navigating the steps that will ensure its success?

This seminar is designed to introduce basic technologies, strategies and considerations in recombinant protein production in E. coli, insect and mammalian cells for multiple research and development applications. The seminar supplies a basic toolbox for management of multiple and diverse projects.

Lost in Translation:

The purpose of this chapter is to illuminate common problems when translating academic ideas and IP to startup companies.

One of the most difficult and frustrating aspects of starting a biotech company is how to analyze and transfer the academic findings to a process suitable for the biotech world. This procedure is often frustrating to both parties and requires psychological skills as well as critical review of the research.

Working in the interphase between academia and industry (on the academic side), we encountered many cases in which good ideas were lost in translation. We discuss several aspects in these interactions, common problems and how to avoid them, and better facilitate this translation, focusing on protein production processes.

1. Avoiding major pitfalls when initiating your startup on academic grounds:

  • Initiating a startup company on campus: pros and cons
  • Precautions and common mistakes

2. Translating research and procedures to biotech language:

  • Reproducibility and technology transfer; research due diligence and early implementation of SOP criteria

3. Working with protein production with CROs and CMOs:

  • QC requirements and process documentation
  • When is it best to do it yourself?

4. Psychology and communication: matching expectations on both ends


5. A series of unfortunate events: case studies and examples

Additional Topics:

  • Overview of recombinant protein production: initiating a project, design and options for various downstream applications, requirements from customer/collaborator, and matching expectations.
  • Introduction to expression systems; covering the differences between the standard expression systems: E. coli, insect cells and mammalian cells, in protein quality, quantity and downstream applications, cost considerations, implementation time, required expertise and more.
  • Basic principles in affinity chromatography, ion exchange, hydrophobic exchange, size exclusion and mixed mode chromatography. Protein purification strategies: input for purification protocol development, guidelines for protein purification, selection and combination of purification techniques.
  • Connection between expression and protein quality; dealing with prone-to-aggregate proteins; selection and combination of purification techniques; major requirements for purification of proteins for structural, biophysical and biochemical studies; minimal quality control parameters and quality control workflow.
  • Troubleshooting and case studies.

Who should attend the training seminar?

The seminar is designed for researchers, lab managers, graduate students, postdocs, technicians and engineers wishing to expand their knowledge and implement basic and advanced technologies in recombinant protein production in their work.

Instructor Biographies:

Tsafi_DanieliTsafi Danieli, Ph.D., Director, BioGiv Excubator & Head, Protein Expression Facility, Wolfson Centre for Applied Structural Biology, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem

Tsafi Danieli is the head of the Protein Expression Core Facility at the Wolfson Centre for Applied Structural Biology at the Hebrew University of Jerusalem, Israel. The core facility functions as a biotechnology research center for the development and implementation of novel technologies in recombinant DNA, molecular biology and protein production. It also operates as a training centre supporting individual research projects and conducting workshops and courses for scientists from academic institutes and from the Biomed & Biotech industry. Dr. Danieli is also the founder and director of “BioGiv”, a specialised university-based center for promoting and supporting entrepreneurs and inventors to translate ideas into new products and bring new technologies to market, while using the infrastructure of the Hebrew University in Jerusalem. Dr. Danieli holds a BSc in Biology and MSc in human genetics from Tel Aviv University, and a Ph.D. in molecular virology from the University of California, San Francisco Pharmacology Department, in collaboration with the Biochemistry Department at Tel Aviv University.

Mario_LebendikerMario Lebendiker, Ph.D., Head, Protein Purification Facility, Wolfson Centre for Applied Structural Biology, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem

Dr. Mario Lebendiker is in charge of the Protein Purification Facility at the Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem. He is actively involved in many collaborations for structural and biochemical studies within the Hebrew University, others Universities in Israel, as well as with biotech and pharmaceutical companies. Dr. Lebendiker received a Ph.D. in Biochemistry in 1982 from the Animal Virology Center (CEVAN), in Buenos Aires University, Argentina. Together with many other laboratories, he found the Protein Production and Purification Partnership in Europe (P4EU) network, a platform for the exchange of information, knowhow and materials between core facility labs in the field of protein expression and purification.

Training Seminar Information

Each CHI Training Seminar offers 1.5 days of instruction with start and stop times for each day shown above and on the Event-at-a-Glance published in the onsite Program & Event Guide. Training Seminars will include morning and afternoon refreshment breaks, as applicable, and lunch will be provided to all registered attendees on the full day of the class.

Each person registered specifically for the training seminar will be provided with a hard copy handbook for the seminar in which they are registered. A limited number of additional handbooks will be available for other delegates who wish to attend the seminar, but after these have been distributed, no additional books will be available.

Though CHI encourages track hopping between conference programs, we ask that Training Seminars not be disturbed once they have begun. In the interest of maintaining the highest quality learning environment for Training Seminar attendees, and because Seminars are conducted differently than conference programming, we ask that attendees commit to attending the entire program, and not engage in track hopping, as to not disturb the hands-on style instruction being offered to the other participants.


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